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验证数据展示

  • WB analysis using 87156-1-RR (same clone as 87156-1-PBS)

    WB analysis using 87156-1-RR (same clone as 87156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.

  • WB analysis using 87156-1-RR (same clone as 87156-1-PBS)

    WB analysis using 87156-1-RR (same clone as 87156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)

    IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)

    IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87156-1-RR (same clone as 87156-1-PBS)

    IF Staining of HeLa using 87156-1-RR (same clone as 87156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS8 antibody (87156-1-RR, Clone: 252267D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.

  • Affinity of 87156-1-RR

    Affinity of 87156-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87156-1-RR against Human NDUFS8 were performed. The affinity constant is below 1 pM.

NDUFS8 Recombinant monoclonal antibody, PBS Only

NDUFS8 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87156-1-PBS

产品说明书

CloneNo. 252267D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87156-1-PBS targets NDUFS8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18531

Product name: Recombinant human NDUFS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC119754

Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
别名 CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2
计算分子量 210 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC119754
基因名称 NDUFS8
Gene ID (NCBI) 4728
RRIDAB_3745377
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00217
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).

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