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验证数据展示

  • WB analysis of A549 using 15728-1-AP (same clone as 15728-1-PBS)

    WB analysis of A549 using 15728-1-AP (same clone as 15728-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS7 antibody included
  • IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS7 antibody included
  • IF Staining of HeLa using 15728-1-AP (same clone as 15728-1-PBS)

    IF Staining of HeLa using 15728-1-AP (same clone as 15728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS7 antibody (15728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

NDUFS7 Polyclonal antibody, PBS Only

NDUFS7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15728-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NDUFS7 Ready-To-Use IHC Kit. NDUFS7 primary antibody included.

产品信息

15728-1-PBS targets NDUFS7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8471

Product name: Recombinant human NDUFS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC005954

Sequence: MAVLSAPGLRGFRILGLRSSVGLAVQARGVHQSVATDGPSSTQPALPKARAVAPKPSSRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALLYGILQLQRKIKRERRLQIWYRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
别名
计算分子量 213 aa, 24 kDa
观测分子量 20 kDa
GenBank蛋白编号BC005954
基因名称 NDUFS7
Gene ID (NCBI) 374291
RRIDAB_2149029
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75251
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS7(NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial) protein is one of the most conserved subunits of mitochondrial respiratory chain complex I and plays a central role in the interaction with the electron acceptor ubiquinone and in the proton-translocating mechanism(PMID:15269216).Defects in NDUFS7 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

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