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验证数据展示

  • WB analysis of rabbit heart using 68329-1-Ig (same clone as 68329-1-PBS)

    WB analysis of rabbit heart using 68329-1-Ig (same clone as 68329-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    IF Staining of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    WB analysis of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

NDUFS6 Monoclonal antibody, PBS Only

NDUFS6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68329-1-PBS

产品说明书

CloneNo. 2F4H3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit, chicken

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2F4H3, CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68329-1-PBS targets NDUFS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit, chicken
免疫原

CatNo: Ag6286

Product name: Recombinant human NDUFS6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-124 aa of BC046155

Sequence: MAAAMTFCRLLNRCGEAARSLPLGARCFGVRVSPTGEKVTHTGQVYDDKDYRRIRFVGRQKEVNENFAIDLIAEQPVSEVETRVIACDGGGGALGHPKVYINLDKETKTGTCGYCGLQFRQHHH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
别名 2F4H3, CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A
计算分子量 14 kDa
观测分子量13-15 kDa
GenBank蛋白编号BC046155
基因名称 NDUFS6
Gene ID (NCBI) 4726
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75380
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

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