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验证数据展示

  • WB analysis of rabbit heart using 68329-1-Ig

    WB analysis of rabbit heart using 68329-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 68329-1-Ig

    IHC staining of human prostate cancer using 68329-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 68329-1-Ig

    IHC staining of human prostate cancer using 68329-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68329-1-Ig

    IF Staining of HepG2 using 68329-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • WB analysis of HepG2 using 68329-1-Ig

    WB analysis of HepG2 using 68329-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

NDUFS6 Monoclonal antibody

NDUFS6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68329-1-Ig

产品说明书

CloneNo. 2F4H3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Rabbit, Chicken

应用

WB, IHC, IF/ICC, ELISA

别名

CI 13kD A, Complex I 13kD A, NDUFS6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrabbit heart tissue, HepG2 cells, rat heart tissue, mouse heart tissue, chicken heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68329-1-Ig targets NDUFS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Rabbit, Chicken
免疫原 NDUFS6 fusion protein Ag6286 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
别名 CI 13kD A, Complex I 13kD A, NDUFS6
计算分子量 14 kDa
观测分子量13-15 kDa
GenBank蛋白编号BC046155
基因名称 NDUFS6
Gene ID (NCBI) 4726
RRIDAB_2935401
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75380
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

实验方案

Product Specific Protocols
WB protocol for NDUFS6 antibody 68329-1-IgDownload protocol
IHC protocol for NDUFS6 antibody 68329-1-IgDownload protocol
IF protocol for NDUFS6 antibody 68329-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols