验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, K-562 cells |
| Positive IHC detected in | human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NDUFAF4 Ready-To-Use IHC Kit. NDUFAF4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26003-1-AP targets NDUFAF4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | NDUFAF4 fusion protein Ag23365 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 |
| 别名 | C6orf66, HRPAP20, HSPC125, My013, NDUFAF4 |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC039464 |
| 基因名称 | NDUFAF4 |
| Gene ID (NCBI) | 29078 |
| RRID | AB_2880329 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P032 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFAF4 is a protein that in humans is encoded by the C6ORF66 gene. NDUFAF4 is an assembly factor of complex I. Mutation of this gene has been associated with congenital disorders of the mitochondrial respiratory chain (PMID: 18179882). NDUFAF4 was previously shown to promote breast cancer cell invasiveness. NDUFAF4 is a 20.2 kDa mitochondrial protein. Catalog#26003-1-AP specifically recognises the 20 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFAF4 antibody 26003-1-AP | Download protocol |
| IHC protocol for NDUFAF4 antibody 26003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
EMBO Rep MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex. | ||
Stem Cell Res Ther Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension. | ||
Virus Genes The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation.
| ||
Cancer Res Commun N-Myristoyltransferase Inhibition causes Mitochondrial Iron Overload and Parthanatos in TIM17A-dependent Aggressive Lung Carcinoma |
