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验证数据展示

  • WB analysis using 15561-1-AP

    WB analysis using 15561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15561-1-AP (NDUFA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 15561-1-AP

    IHC staining of mouse liver using 15561-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15561-1-AP

    IHC staining of mouse liver using 15561-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDUFA1 Polyclonal antibody

NDUFA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15561-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NADH-ubiquinone oxidoreductase MWFE subunit, MWFE, Complex I-MWFE, Complex I MWFE, CI-MWFE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat heart tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15561-1-AP targets NDUFA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 NDUFA1 fusion protein Ag7206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
别名 NADH-ubiquinone oxidoreductase MWFE subunit, MWFE, Complex I-MWFE, Complex I MWFE, CI-MWFE
计算分子量7.5 kDa
观测分子量7.5 kDa
GenBank蛋白编号BC000266
基因名称 NDUFA1
Gene ID (NCBI) 4694
RRIDAB_2918040
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15239
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The NDUFA1 gene encoding the MWFE polypeptide is located on the X chromosome. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria, and it is predicted to be a membrane protein (PMID: 10200266).

实验方案

Product Specific Protocols
WB protocol for NDUFA1 antibody 15561-1-APDownload protocol
IHC protocol for NDUFA1 antibody 15561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Endocrinol (Lausanne)

Changes and related factors of blood CCN1 levels in diabetic patients

Authors - Zhao-Yu Xiang
View Article
humanWB

Methods Mol Biol

Solvent-Induced Protein Precipitation for Drug Target Discovery

Authors - Xiaolei Zhang
View Article