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验证数据展示

  • WB analysis using 33254-1-AP

    WB analysis using 33254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33254-1-AP (NDRG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NDRG4 Polyclonal antibody

NDRG4 Polyclonal Antibody for WB, ELISA
Cat No. 33254-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

BDM1, Brain development-related molecule 1, NDRG family member 4, N-myc downstream-regulated gene 4 protein, Protein NDRG4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy® NDRG4 Ready-To-Use IHC Kit. NDRG4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33254-1-AP targets NDRG4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag38351

Product name: Recombinant human NDRG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-223 aa of BC011795

Sequence: NHKLCFNTFFNFEDMQEITKHFVVCHVDAPGQQVGASQFPQGYQFPSMEQLAAMLPSVVQHFGFKYVIGIGVGAGAYVLAKFALIFPDLVEGLVLVNIDPNGKGWIDWAATKLSGLTSTLPDTVLSHLFSQEELVNNTELVQSYRQQIGNVVNQANLQLFWNMYNSRRDLDINRPGTVPNAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NDRG family member 4
别名 BDM1, Brain development-related molecule 1, NDRG family member 4, N-myc downstream-regulated gene 4 protein, Protein NDRG4
计算分子量 339 aa, 37 kDa
观测分子量37 kDa, 38 kDa and 41 kDa
GenBank蛋白编号BC011795
基因名称 NDRG4
Gene ID (NCBI) 65009
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9ULP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDRG4 (N-myc downstream-regulated gene 4) is a member of the NDRG family (NDRG1-4), a group of evolutionarily conserved proteins implicated in cell differentiation, stress responses, and tumor suppression. Unlike other family members (e.g., NDRG1 linked to cancer metastasis), NDRG4 is highly expressed in the brain and heart, with emerging roles in neuronal development and cardiovascular function. Methylation of NDRG4 in stool DNA is a diagnostic marker for colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for NDRG4 antibody 33254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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