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验证数据展示

  • WB analysis using 83902-7-RR

    WB analysis using 83902-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83902-7-RR (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83902-7-RR

    WB analysis of HepG2 using 83902-7-RR

    WB result of NDRG2 antibody (83902-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG2 transfected HepG2 cells.

  • FC experiment of HepG2 using 83902-7-RR

    FC experiment of HepG2 using 83902-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDRG2 Recombinant antibody (83902-7-RR, Clone:241039E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse brain using 83902-7-RR

    IHC staining of mouse brain using 83902-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included
  • IHC staining of mouse brain using 83902-7-RR

    IHC staining of mouse brain using 83902-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDRG2 antibody included
  • Affinity and Kinetic Characterization of 83902-7-RR

    Affinity and Kinetic Characterization of 83902-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83902-7-RR against Human NDRG2 were performed. The affinity constant is 0.114 nM.

  • Featured Product
  • KD/KO验证

NDRG2 Recombinant antibody

NDRG2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83902-7-RR

产品说明书

CloneNo. 241039E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

241039E8, NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue, mouse heart tissue, rat heart tissue, human heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NDRG2 Ready-To-Use IHC Kit. NDRG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83902-7-RR targets NDRG2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDRG2 fusion protein Ag2629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NDRG family member 2
别名 241039E8, NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613
计算分子量 371 aa, 39 kDa
观测分子量41-45 kDa
GenBank蛋白编号BC010458
基因名称 NDRG2
Gene ID (NCBI) 57447
RRIDAB_3671484
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UN36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-myc downstream-regulated gene 2 (NDRG2) is a newly identified member of the NDRG family that is highly localized and plays an important role in the regulation of astrocyte function. In astrocytes, NDRG2 affects the regulation of apoptosis, astrogliosis, blood-brain barrier integrity, and glutamate clearance. Several preclinical studies have revealed that NDRG2 is implicated in the pathogenesis of many neurological diseases, such as stroke, neurodegeneration and psychiatric disorders. Recent studies demonstrated that NDRG2 functions as a tumor suppressor and may be a prognostic predictor for many different malignant tumors.

实验方案

Product Specific Protocols
WB protocol for NDRG2 antibody 83902-7-RRDownload protocol
IHC protocol for NDRG2 antibody 83902-7-RRDownload protocol
FC protocol for NDRG2 antibody 83902-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols