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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 83902-7-RR (same clone as 83902-7-PBS)

    WB analysis using 83902-7-RR (same clone as 83902-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83902-7-RR (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83902-7-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83902-7-RR (same clone as 83902-7-PBS)

    WB analysis of HepG2 using 83902-7-RR (same clone as 83902-7-PBS)

    WB result of NDRG2 antibody (83902-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG2 transfected HepG2 cells. This data was developed using the same antibody clone with 83902-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83902-7-RR (same clone as 83902-7-PBS)

    FC experiment of HepG2 using 83902-7-RR (same clone as 83902-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDRG2 Recombinant antibody (83902-7-RR, Clone:241039E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83902-7-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83902-7-RR (same clone as 83902-7-PBS)

    IHC staining of mouse brain using 83902-7-RR (same clone as 83902-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83902-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDRG2 antibody included
  • IHC staining of mouse brain using 83902-7-RR (same clone as 83902-7-PBS)

    IHC staining of mouse brain using 83902-7-RR (same clone as 83902-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83902-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDRG2 antibody included
  • Affinity and Kinetic Characterization of 83902-7-RR

    Affinity and Kinetic Characterization of 83902-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83902-7-RR against Human NDRG2 were performed. The affinity constant is 0.114 nM.

  • Featured Product
  • KD/KO验证

NDRG2 Recombinant antibody, PBS Only

NDRG2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83902-7-PBS

产品说明书

CloneNo. 241039E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

241039E8, NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NDRG2 Ready-To-Use IHC Kit. NDRG2 primary antibody included.

产品信息

83902-7-PBS targets NDRG2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDRG2 fusion protein Ag2629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NDRG family member 2
别名 241039E8, NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613
计算分子量 371 aa, 39 kDa
观测分子量41-45 kDa
GenBank蛋白编号BC010458
基因名称 NDRG2
Gene ID (NCBI) 57447
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UN36
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-myc downstream-regulated gene 2 (NDRG2) is a newly identified member of the NDRG family that is highly localized and plays an important role in the regulation of astrocyte function. In astrocytes, NDRG2 affects the regulation of apoptosis, astrogliosis, blood-brain barrier integrity, and glutamate clearance. Several preclinical studies have revealed that NDRG2 is implicated in the pathogenesis of many neurological diseases, such as stroke, neurodegeneration and psychiatric disorders. Recent studies demonstrated that NDRG2 functions as a tumor suppressor and may be a prognostic predictor for many different malignant tumors.