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验证数据展示

  • WB analysis using 86167-1-RR

    WB analysis using 86167-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86167-1-RR (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86167-1-RR

    IF Staining of HeLa using 86167-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDP52 antibody (86167-1-RR, Clone: 250714E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86167-1-RR

    Affinity and Kinetic Characterization of 86167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86167-1-RR against Human NDP52 were performed. The affinity constant is 0.643 nM.

NDP52 Recombinant monoclonal antibody

NDP52 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86167-1-RR

产品说明书

CloneNo. 250714E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, A549 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86167-1-RR targets NDP52 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2866

Product name: Recombinant human CALCOCO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC015893

Sequence: MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium binding and coiled-coil domain 2
别名 CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52
计算分子量 446 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC015893
基因名称 NDP52
Gene ID (NCBI) 10241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13137
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa.

实验方案

Product Specific Protocols
IF protocol for NDP52 antibody 86167-1-RRDownload protocol
WB protocol for NDP52 antibody 86167-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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