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验证数据展示

  • WB analysis using 86167-1-RR (same clone as 86167-1-PBS)

    WB analysis using 86167-1-RR (same clone as 86167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86167-1-RR (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86167-1-RR (same clone as 86167-1-PBS)

    IF Staining of HeLa using 86167-1-RR (same clone as 86167-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDP52 antibody (86167-1-RR, Clone: 250714E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86167-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86167-1-RR

    Affinity and Kinetic Characterization of 86167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86167-1-RR against Human NDP52 were performed. The affinity constant is 0.643 nM.

NDP52 Recombinant monoclonal antibody, PBS Only

NDP52 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86167-1-PBS

产品说明书

CloneNo. 250714E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86167-1-PBS targets NDP52 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2866

Product name: Recombinant human CALCOCO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC015893

Sequence: MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium binding and coiled-coil domain 2
别名 CALCOCO2, Calcium-binding and coiled-coil domain-containing protein 2, Nuclear domain 10 protein 52, Nuclear dot protein 52
计算分子量 446 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC015893
基因名称 NDP52
Gene ID (NCBI) 10241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13137
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa.

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