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验证数据展示

  • WB analysis using CL488-10506

    WB analysis using CL488-10506

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-10506 (NCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using CL488-10506

    FC experiment of SH-SY5Y using CL488-10506

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NCS1 (CL488-10506) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NCS1 Polyclonal antibody

NCS1 Polyclonal Antibody for WB, FC (Intra)
Cat No. CL488-10506

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10506 targets NCS1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0784

Product name: Recombinant human FREQ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC004856

Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 frequenin homolog (Drosophila)
别名 FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC004856
基因名称 NCS1
Gene ID (NCBI) 23413
RRIDAB_3672448
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62166
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCS1 is a member of the neuronal calcium sensor gene family. It regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 NCS1 antibody CL488-10506Download protocol
Standard Protocols
Click here to view our Standard Protocols
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