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验证数据展示

  • WB analysis using 20175-1-AP (same clone as 20175-1-PBS)

    WB analysis using 20175-1-AP (same clone as 20175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    IP experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    IP result of anti-NCOA5 (IP:20175-1-AP, 4ug; Detection:20175-1-AP 1:800) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 20175-1-AP (same clone as 20175-1-PBS)

    IHC staining of human stomach using 20175-1-AP (same clone as 20175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20175-1-AP (NCOA5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    FC experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Polyclonal antibody (20175-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 20175-1-AP (same clone as 20175-1-PBS)

    IF Staining of A431 using 20175-1-AP (same clone as 20175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (20175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NCOA5 Polyclonal antibody, PBS Only

NCOA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20175-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5, Nuclear receptor coactivator 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20175-1-PBS targets NCOA5 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14014

Product name: Recombinant human NCOA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 112-384 aa of BC056872

Sequence: DMRDSRDPMYRREGSYDRYLRMDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRQYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor coactivator 5
别名 CIA, Coactivator independent of AF-2, KIAA1637, NCoA-5, Nuclear receptor coactivator 5
计算分子量 579 aa, 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC056872
基因名称 NCOA5
Gene ID (NCBI) 57727
RRIDAB_10694674
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCD5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).

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