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验证数据展示

  • WB analysis using 29933-1-AP

    WB analysis using 29933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29933-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 29933-1-AP

    WB analysis of HEK-293 using 29933-1-AP

    WB result of NCAPG antibody (29933-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPG transfected HEK-293 cells.

  • IHC staining of human ovary tumor using 29933-1-AP

    IHC staining of human ovary tumor using 29933-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29933-1-AP (NCAPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPG antibody included
  • Featured Product
  • KD/KO验证

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29933-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CAPG, CHCG, Chromosome-associated protein G, Condensin complex subunit 3, Condensin subunit CAP G

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPG Ready-To-Use IHC Kit. CAPG primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29933-1-AP targets NCAPG in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32040

Product name: Recombinant human NCAPG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 870-1015 aa of BC068467

Sequence: LLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit G
别名 CAPG, CHCG, Chromosome-associated protein G, Condensin complex subunit 3, Condensin subunit CAP G
计算分子量 1015 aa, 114 kDa
观测分子量131 kDa
GenBank蛋白编号BC068467
基因名称 NCAPG
Gene ID (NCBI) 64151
RRIDAB_2923621
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BPX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for NCAPG antibody 29933-1-APDownload protocol
WB protocol for NCAPG antibody 29933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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