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验证数据展示

  • WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

    WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

    IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

    Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

    IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAPD2 antibody included
  • IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

    IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAPD2 antibody included
  • Featured Product
  • KD/KO Validated

NCAPD2 Polyclonal antibody, PBS Only

NCAPD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13382-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCAPD2 Ready-To-Use IHC Kit. NCAPD2 primary antibody included.

产品信息

13382-1-PBS targets NCAPD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4219

Product name: Recombinant human NCAPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC028182

Sequence: MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLEFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit D2
别名 Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2
计算分子量 1401 aa, 157 kDa
观测分子量 157 kDa
GenBank蛋白编号BC028182
基因名称 NCAPD2
Gene ID (NCBI) 9918
RRIDAB_2149840
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15021
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.

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