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验证数据展示

  • WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

    WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

    IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

    IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAPD2 antibody included
  • IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

    IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAPD2 antibody included
  • Featured Product
  • KD/KO Validated

NCAPD2 Polyclonal antibody, PBS Only

NCAPD2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13382-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CAP D2, CAPD2, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, CNAP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCAPD2 Ready-To-Use IHC Kit. NCAPD2 primary antibody included.

产品信息

13382-1-PBS targets NCAPD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4219

Product name: Recombinant human NCAPD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC028182

Sequence: MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLEFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC condensin I complex, subunit D2
别名 CAP D2, CAPD2, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, CNAP1
计算分子量 1401 aa, 157 kDa
观测分子量 157 kDa
GenBank蛋白编号BC028182
基因名称 NCAPD2
Gene ID (NCBI) 9918
RRIDAB_2149840
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15021
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.

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