验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells, mouse cerebellum tissue, human brain tissue, human heart tissue, human cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12925-1-AP targets NCALD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3614 Product name: Recombinant human NCALD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC036098 Sequence: MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSMEEFIRGAKSDPSIVRLLQCDPSSAGQF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurocalcin delta |
| 别名 | NCALD, neurocalcin delta, neurocalcin δ |
| 计算分子量 | 193 aa, 22 kDa |
| 观测分子量 | 18-22 kDa |
| GenBank蛋白编号 | BC036098 |
| 基因名称 | NCALD |
| Gene ID (NCBI) | 83988 |
| RRID | AB_2149410 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61601 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCALD belongs to the recoverin family. And it is a member of the EF-hand calcium-binding protein superfamily. NCALD may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. It plays an important role in the regulation of the neuronal signal transduction process. NCALD is a likely candidate for conferring susceptibility to diabetic nephropathy.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NCALD antibody 12925-1-AP | Download protocol |
| WB protocol for NCALD antibody 12925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone. | ||
Am J Hum Genet Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.
| ||
Oncotarget Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer. | ||
Int J Oncol N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer. | ||
Front Neurol Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. | ||
Biomark Med NCALD as a potential predictive biomarker for the efficacy of platinum-based chemotherapy in ovarian cancer |
