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验证数据展示

  • WB analysis using 23841-1-AP (same clone as 23841-1-PBS)

    WB analysis using 23841-1-AP (same clone as 23841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23841-1-AP (NAT8L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23841-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 23841-1-AP (same clone as 23841-1-PBS)

    IF Staining of HEK-293 using 23841-1-AP (same clone as 23841-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NAT8L antibody (23841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23841-1-PBS in a different storage buffer formulation.

NAT8L Polyclonal antibody, PBS Only

NAT8L Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23841-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23841-1-PBS targets NAT8L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20832

Product name: Recombinant human NAT8L protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-134 aa of BC103748

Sequence: MADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRKVLEFAVVHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 8-like (GCN5-related, putative)
别名 NAA synthetase, Hcml3, EC:2.3.1.17, CML3, Camello-like protein 3
计算分子量 134 aa, 15 kDa
观测分子量48 kDa
GenBank蛋白编号BC103748
基因名称 NAT8L
Gene ID (NCBI) 339983
RRIDAB_3669418
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8N9F0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NAT8L (N-acetyltransferase 8-like) catalyzes the formation of N-acetyl aspartate (NAA) from acetyl-CoA and aspartate. In the brain, NAA delivers the acetate moiety to synthesize acetyl-CoA, which is further used for fatty acid generation (PMID: 24155240). NAT8L modulation may impinge on the metabolic reprogramming of cancer cells (PMID: 36580805). The calculated MW of NAT8L is around 33 kDa, and the observed MW is around 45 kDa.

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