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  • Featured Product
  • KD/KO Validated

NAT6/FUS2 Polyclonal antibody

NAT6/FUS2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15476-1-AP

Print datasheet

Synonyms

FUS 2, FUS2, N acetyltransferase 6, NAA80, NAT6, Protein fus 2, Protein fusion 2

验证数据展示

  • WB analysis using 15476-1-AP

    WB analysis using 15476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15476-1-AP (NAT6/FUS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15476-1-AP

    IF Staining of HeLa using 15476-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAT6/FUS2 antibody (15476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15476-1-AP targets NAT6/FUS2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen NAT6/FUS2 fusion protein Ag7780 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 6 (GCN5-related)
Synonyms FUS 2, FUS2, N acetyltransferase 6, NAA80, NAT6, Protein fus 2, Protein fusion 2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC004483
Gene Symbol NAT6
Gene ID (NCBI) 24142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93015
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The acetyltransferase NAT6 (also known as Fus2) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines and located mainly in the cytoplasm. NAT6 has two isoforms. The molecular weight of NAT6 was detected to be about 45kDa (PMID:32978259).

实验方案

Product Specific Protocols
WB protocol for NAT6/FUS2 antibody 15476-1-APDownload protocol
IF protocol for NAT6/FUS2 antibody 15476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

FEBS J

NAT6 acetylates the N-terminus of different forms of actin.

Authors - Elsa Wiame
  • KO Validated
View Article
humanWB

Brain Commun

NAA80 bi-allelic missense variants result in high-frequency hearing loss, muscle weakness and developmental delay.

Authors - Irena J J Muffels
View Article