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NAT2 Polyclonal antibody

NAT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11410-1-AP

Print datasheet

Synonyms

AAC2, Arylamide acetylase 2, N acetyltransferase type 2, NAT 2, NAT2, PNAT

验证数据展示

  • WB analysis using 11410-1-AP

    WB analysis using 11410-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 11410-1-AP

    IHC staining of human small intestine using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human breast cancer using 11410-1-AP

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11410-1-AP

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11410-1-AP targets NAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen NAT2 fusion protein Ag1953 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 2 (arylamine N-acetyltransferase)
Synonyms AAC2, Arylamide acetylase 2, N acetyltransferase type 2, NAT 2, NAT2, PNAT
Calculated Molecular Weight 33.5 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC015878
Gene Symbol NAT2
Gene ID (NCBI) 10
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11245
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).

实验方案

Product Specific Protocols
WB protocol for NAT2 antibody 11410-1-APDownload protocol
IHC protocol for NAT2 antibody 11410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols