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NARFL Polyclonal antibody

NARFL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13652-1-AP

Print datasheet

Synonyms

HPRN, IOP1, LET1L, NARFL, PRN, Protein related to Narf

验证数据展示

  • WB analysis using 13652-1-AP

    WB analysis using 13652-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13652-1-AP (NARFL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 13652-1-AP

    IHC staining of human colon using 13652-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 13652-1-AP

    IHC staining of human colon using 13652-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13652-1-AP targets NARFL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NARFL fusion protein Ag4217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear prelamin A recognition factor-like
Synonyms HPRN, IOP1, LET1L, NARFL, PRN, Protein related to Narf
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC030248
Gene Symbol NARFL
Gene ID (NCBI) 64428
RRIDAB_2877965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Q4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NARFL(Nuclear prelamin A recognition factor-like protein) is also named as PRN and belongs to the NARF family. This protein plays a role in cytoplasmic Fe-S protein biogenesis in mammalian cells. NARFL has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NARFL antibody 13652-1-APDownload protocol
IHC protocol for NARFL antibody 13652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Rep

NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α-DNMT1 axis

Authors - Hongzhou Liu
View Article