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验证数据展示

  • IF Staining of human kidney using 86052-4-RR

    IF Staining of human kidney using 86052-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human kidney using 86052-4-RR

    IF Staining of human kidney using 86052-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 86052-4-RR

    IHC staining of human lung using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung using 86052-4-RR

    IHC staining of human lung using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included

Napsin A Recombinant monoclonal antibody

Napsin A Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 86052-4-RR

产品说明书

CloneNo. 242433C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF-P, ELISA

别名

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86052-4-RR targets Napsin A in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9721

Product name: Recombinant human NAPSA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC017842

Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 napsin A aspartic peptidase
别名 NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-
计算分子量 420 aa, 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC017842
基因名称 Napsin A
Gene ID (NCBI) 9476
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO96009
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Napsin A antibody 86052-4-RRDownload protocol
IHC protocol for Napsin A antibody 86052-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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