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验证数据展示

  • WB analysis of mouse lung using 16558-1-AP

    WB analysis of mouse lung using 16558-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16558-1-AP

    IHC staining of human kidney using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung cancer using 16558-1-AP

    IHC staining of human lung cancer using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung cancer using 16558-1-AP

    IHC staining of human lung cancer using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • FC experiment of A549 using 16558-1-AP

    FC experiment of A549 using 16558-1-AP

    1x10^6 A549 cells were intracellularly stained with 4 ug Napsin A Polyclonal antibody (16558-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human kidney using 16558-1-AP

    IHC staining of human kidney using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IF Staining of HUVEC using 16558-1-AP

    IF Staining of HUVEC using 16558-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (16558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Napsin A Polyclonal antibody

Napsin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 16558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 4.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16558-1-AP targets Napsin A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9786

Product name: Recombinant human NAPSA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC017842

Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 napsin A aspartic peptidase
别名 NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-
计算分子量 420 aa, 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC017842
基因名称 Napsin A
Gene ID (NCBI) 9476
RRIDAB_2878278
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96009
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

实验方案

Product Specific Protocols
FC protocol for Napsin A antibody 16558-1-APDownload protocol
IF protocol for Napsin A antibody 16558-1-APDownload protocol
IHC protocol for Napsin A antibody 16558-1-APDownload protocol
WB protocol for Napsin A antibody 16558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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