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Napsin A Polyclonal antibody

Napsin A Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16558-1-AP

Print datasheet

Synonyms

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

验证数据展示

  • WB analysis of mouse lung using 16558-1-AP

    WB analysis of mouse lung using 16558-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16558-1-AP

    IHC staining of human lung cancer using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IHC staining of human lung cancer using 16558-1-AP

    IHC staining of human lung cancer using 16558-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Napsin A antibody included
  • IF Staining of PC-3 using 16558-1-AP

    IF Staining of PC-3 using 16558-1-AP

    Immunofluorescent analysis of PC-3 cells using 16558-1-AP (Napsin A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16558-1-AP targets Napsin A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen Napsin A fusion protein Ag9786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name napsin A aspartic peptidase
Synonyms Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02
Calculated Molecular Weight 420 aa, 45 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC017842
Gene Symbol NAPSA
Gene ID (NCBI) 9476
RRIDAB_2878278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96009
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

实验方案

Product Specific Protocols
WB protocol for Napsin A antibody 16558-1-APDownload protocol
IHC protocol for Napsin A antibody 16558-1-APDownload protocol
IF protocol for Napsin A antibody 16558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols