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验证数据展示

  • WB analysis of mouse testis using 21679-1-AP (same clone as 21679-1-PBS)

    WB analysis of mouse testis using 21679-1-AP (same clone as 21679-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21679-1-AP (NANOS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 21679-1-AP (same clone as 21679-1-PBS)

    WB analysis of HEK-293 using 21679-1-AP (same clone as 21679-1-PBS)

    WB result of NANOS3 antibody (21679-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NANOS3 transfected HEK-293 cells. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NANOS3 Polyclonal antibody, PBS Only

NANOS3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21679-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

NOS-3, NOS3, NOS 3, NANOS1L, nanos homolog 3 (Drosophila)

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21679-1-PBS targets NANOS3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16014

Product name: Recombinant human NANOS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC101209

Sequence: MGTFDLWTDYLGLAHLVRALSGKEGPETRLSPQPEPEPMLEPVSALEPMPAPESVPVPGPKDQKRSLESSPAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATRERAHTRRFCPLTGQGYTSVYSHTTRNSAGKKLVRPDKAKTQDTGHRRGGGGGAGFRGAGKSEPSPSCSPSMST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nanos homolog 3 (Drosophila)
别名 NOS-3, NOS3, NOS 3, NANOS1L, nanos homolog 3 (Drosophila)
计算分子量 192 aa, 21 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC101209
基因名称 NANOS3
Gene ID (NCBI) 342977
RRIDAB_10859250
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60323
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.

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