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验证数据展示

  • WB analysis using 82968-1-RR (same clone as 82968-1-PBS)

    WB analysis using 82968-1-RR (same clone as 82968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82968-1-RR (same clone as 82968-1-PBS)

    WB analysis of HEK-293 using 82968-1-RR (same clone as 82968-1-PBS)

    WB result of N-cadherin antibody (82968-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR (same clone as 82968-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR (same clone as 82968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of A549 using 82968-1-RR (same clone as 82968-1-PBS)

    IF Staining of A549 using 82968-1-RR (same clone as 82968-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    IF Staining of HeLa using 82968-1-RR (same clone as 82968-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82968-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82968-1-RR

    Affinity and Kinetic Characterization of 82968-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

  • Featured Product
  • KD/KO验证

N-cadherin Recombinant monoclonal antibody, PBS Only

N-cadherin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82968-1-PBS

产品说明书

CloneNo. 230259H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

230259H5, Cadherin 2, Cadherin-2, CD325, CDH2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

82968-1-PBS targets N-cadherin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0701

Product name: Recombinant Human N-Cadherin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 160-724 aa of NM_001792.4

Sequence: DWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 2, type 1, N-cadherin (neuronal)
别名 230259H5, Cadherin 2, Cadherin-2, CD325, CDH2
计算分子量100 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001792.4
基因名称 N-cadherin
Gene ID (NCBI) 1000
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19022-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

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