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验证数据展示

  • WB analysis using 82968-1-RR

    WB analysis using 82968-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82968-1-RR

    WB analysis of HEK-293 using 82968-1-RR

    WB result of N-cadherin antibody (82968-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human heart using 82968-1-RR

    IHC staining of human heart using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Heart Muscle slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 40x lens).Heat mediated antigen retrieval with Tris EDTA (pH 9.0) buffer. Staining was performed on the Biocare NeoPATH Pro. The staining was performed on an autostainer.

  • IHC staining of human heart using 82968-1-RR

    IHC staining of human heart using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of Cholangiocarcinoma using 82968-1-RR

    IHC staining of Cholangiocarcinoma using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded Cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 82968-1-RR

    IHC staining of human liver using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 82968-1-RR

    IHC staining of human liver using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human appendicitis using 82968-1-RR

    IHC staining of human appendicitis using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human brain using 82968-1-RR

    IHC staining of human brain using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of Cholangiocarcinoma using 82968-1-RR

    IHC staining of Cholangiocarcinoma using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded Cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human liver cancer using 82968-1-RR

    IHC staining of human liver cancer using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human heart using 82968-1-RR

    IHC staining of human heart using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of A549 using 82968-1-RR

    IF Staining of A549 using 82968-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of HeLa using 82968-1-RR

    IF Staining of HeLa using 82968-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 82968-1-RR

    IF Staining of HeLa using 82968-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82968-1-RR

    Affinity and Kinetic Characterization of 82968-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

  • Featured Product
  • KD/KO Validated

N-cadherin Recombinant monoclonal antibody

N-cadherin Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, ELISA
Cat No. 82968-1-RR

产品说明书

CloneNo. 230259H5
引用文献(9)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IHC-Autostainer, IF/ICC, ELISA

别名

230259H5, Cadherin 2, Cadherin-2, CD325, CDH2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC-Autostainer detected inhuman heart tissue, Cholangiocarcinoma tissue, human liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human appendicitis tissue, human liver cancer tissue, human ovary cancer tissue, human brain tissue, Cholangiocarcinoma tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:50-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

82968-1-RR targets N-cadherin in WB, IHC, IHC-Autostainer, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IHC-Autostainer, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Eg0701

Product name: Recombinant Human N-Cadherin protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 160-724 aa of NM_001792.4

Sequence: DWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 2, type 1, N-cadherin (neuronal)
别名 230259H5, Cadherin 2, Cadherin-2, CD325, CDH2
计算分子量100 kDa
观测分子量130 kDa
GenBank蛋白编号NM_001792.4
基因名称 N-cadherin
Gene ID (NCBI) 1000
RRIDAB_3670715
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19022-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

实验方案

Product Specific Protocols
IF protocol for N-cadherin antibody 82968-1-RRDownload protocol
IHC protocol for N-cadherin antibody 82968-1-RRDownload protocol
WB protocol for N-cadherin antibody 82968-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Microbiol

Bacteroides fragilis toxin promotes gall bladder cancer in mice.

Authors - Jun Chen
View Article

Front Immunol

Prognostic significance and immune infiltration analysis of HMGA2 in endometrial cancer

Authors - Peng Jiang
View Article
humanWB

Front Immunol

The prognostic significance of JAML and its role in remodeling the immune microenvironment via the cGAS-STING pathway in endometrial cancer.

Authors - Chenfan Tian
View Article
humanWB

Eur J Med Res

The S1PR1-CCN1 axis drives endothelial-to-mesenchymal transition and vascular instability in brain arteriovenous malformations.

Authors - Ganglei Li
View Article

J Cereb Blood Flow Metab

EXPRESS: NSUN6-mediated 5-methylcytosine modification of USP2-AS1 drives Wnt/β-catenin signaling in brain arteriovenous malformations.

Authors - Ganglei Li
View Article
humanWB

Biomedicines

Inhibition of TPI1 Sensitizes Cisplatin-Resistant Oral Cancer to Ferroptosis

Authors - Dandan Wang
View Article
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