Validation Data Gallery
Product Information
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads. • Fast, reliable & efficient one-step immunoprecipitation • Elution with peptide • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Binding capacity | 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | 2x Myc peptide. SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 1x Myc-tag: Dissociation constant KD of 500 nM 2x Myc-tag: Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631369 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Publications
| Application | Title |
|---|---|
Cell Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis. | |
Nat Genet MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding. | |
J Hepatol Immunity-related GTPase induces lipophagy to prevent excess hepatic lipid accumulation. | |
Nat Neurosci TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration. | |
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. |