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CoraLite®594-conjugated Mu Crystallin Polyclonal antibody

Mu Crystallin Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-12495

Print datasheet

Synonyms

CRYM, crystallin, mu, DFNA40, Mu Crystallin, Mu crystallin homolog, THBP

验证数据展示

  • IF Staining of human gliomas using CL594-12495

    IF Staining of human gliomas using CL594-12495

    Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using CoraLite®594 Mu Crystallin antibody (CL594-12495) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL594-12495

    FC experiment of SH-SY5Y using CL594-12495

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Mu Crystallin (CL594-12495) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman gliomas tissue
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 12495-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-12495 targets Mu Crystallin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Immunogen Mu Crystallin fusion protein Ag3161 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crystallin, mu
Synonyms CRYM, crystallin, mu, DFNA40, Mu Crystallin, Mu crystallin homolog, THBP
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC018061
Gene Symbol CRYM
Gene ID (NCBI) 1428
RRIDAB_2919784
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14894
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

实验方案

Product Specific Protocols
IF protocol for CL594 Mu Crystallin antibody CL594-12495Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cell Stem Cell

SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS

Authors - Gabriel R Linares
View Article