• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Biotin-conjugated Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC

Conjugate

Biotin

Cat No : Biotin-10283

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human colon cancer using Biotin-10283

    IHC staining of human colon cancer using Biotin-10283

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (alpha tubulin antibody) and Biotin-10283 (Biotin-Rabbit anti-Mouse IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-10283 targets Mouse-IgG in IHC applications and shows reactivity with mouse samples.

Tested Applications IHC
Tested Reactivity mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mouse-IgG
Synonyms
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 25 kDa, 50-55 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918932
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detectes all the heavy and light chains of total mouse IgG.

实验方案

Product Specific Protocols
IHC protocol for Biotin Mouse-IgG antibody Biotin-10283Download protocol
Standard Protocols
Click here to view our Standard Protocols