Mouse CTLA-4 ELISA Kit
Sensitivity
2.3 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10177 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CTLA-4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CTLA-4. The assay recognizes mouse CTLA-4. An antibody specific for mouse CTLA-4 has been pre-coated onto the microwells. The mouse CTLA-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CTLA-4 is added to detect the captured mouse CTLA-4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CTLA-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12477 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 74% | 70%-77% |
| Tissue lysate | 93% | 85%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 850.4 | 29.6 | 3.5 |
| 2 | 8 | 177.4 | 8.2 | 4.6 |
| 3 | 8 | 92.5 | 7.6 | 8.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 800.1 | 60.7 | 7.6 |
| 2 | 16 | 181.3 | 9.1 | 5.0 |
| 3 | 16 | 91.7 | 7.5 | 8.1 |
Background Information
Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) is a co-inhibitory receptor that plays a crucial role in regulating T-cell activation and maintaining immune tolerance and delivers inhibitory signals that suppress T-cell responses, thereby preventing autoimmune diseases. It is expressed on activated T cells and regulatory T cells (Tregs), which mediates its inhibitory function by competing with CD28 for binding to CD80/CD86, depleting these ligands from antigen-presenting cells (APCs) and dendritic cells (DCs), and delivering inhibitory signals via its intracellular tail. CTLA-4 exists in two forms: membrane-bound CTLA-4 (mCTLA-4) and soluble CTLA-4 (sCTLA-4). mCTLA-4 is a transmembrane protein localized on the surface of T cells and Tregs, where it interacts with CD80 and CD86 on APCs. sCTLA-4 lacks the transmembrane domain and is released into the extracellular environment, where it can modulate immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ctla4, Cd152, Ctla 4, Ly 56 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
