Speedy™ Mouse BAFF One-Step ELISA Kit
Sensitivity
15.6 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Mouse
Cat no : SE60006
Synonyms
B-cell-activating factor, CD257, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, Tumor necrosis factor ligand superfamily member 13b, membrane form
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse BAFF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 15.6 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24099 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 117% | 107%-126% |
| Cell culture supernatant | 91% | 75%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,739.0 | 77.4 | 4.5 |
| 2 | 8 | 426.0 | 20.9 | 4.9 |
| 3 | 8 | 226.0 | 16.6 | 7.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,724.0 | 104.0 | 6.0 |
| 2 | 16 | 428.9 | 21.5 | 5.0 |
| 3 | 16 | 225.8 | 13.1 | 5.8 |
Background Information
TNFSF13B, also named as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13B is localized in chromosome region 13q32-q34 and is implicated in the regulation of immune response to infections, autoimmune disease, and inflammation. BAFF is a critical cytokine supporting the survival of naive B cells, acting through the BAFFR receptor. At the placental level, it is believed that TNFSF13B has an antiapoptotic effect. TNFSF13B is overexpressed in several autoimmune patients although the mechanism behind this feature is unclear.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell-activating factor, CD257, Tnfsf13b, Tumor necrosis factor ligand superfamily member 13B, Tumor necrosis factor ligand superfamily member 13b, membrane form |
FAQ
1、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
