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验证数据展示

  • Dot Blot experiment of peptide using 82825-2-RR

    Dot Blot experiment of peptide using 82825-2-RR

    Dot blot analysis was used to confirm the specificity of Mono-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis using 82825-2-RR

    WB analysis using 82825-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82825-2-RR

    IF Staining of HeLa using 82825-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys36) antibody (82825-2-RR, Clone: 241138B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 82825-2-RR

    FC experiment of HeLa using 82825-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Mono-Methyl-Histone H3 (Lys36) Recombinant antibody

Mono-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, ELISA
Cat No. 82825-2-RR

产品说明书

CloneNo. 241138B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Dot Blot, ELISA

别名

HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, THP-1 cells, K-562 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82825-2-RR targets Mono-Methyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3670565
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

实验方案

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RRDownload protocol
IF protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols