Mono/Di-Methyl-Histone H3 (Lys9) Recombinant antibody
Mono/Di-Methyl-Histone H3 (Lys9) Recombinant Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E12
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells |
Positive IF detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:5000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80219-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, WB, ELISA |
Tested Reactivity | Human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | histone cluster 1, H3a |
Synonyms | di-methylated histone H3, H3K9me1, H3K9me1/2, H3K9me2, histone, Mono/Di-Methyl-Histone H3 (Lys9), mono-methylated histone H3, monomethyl-Histone H3 |
Observed Molecular Weight | 15-18 kDa |
GenBank Accession Number | BC066245 |
Gene Symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_2918878 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P68431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR | Download protocol |
IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |