验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HSC-T6 cells, 4T1 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
30145-1-AP targets MLKL in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag32514 Product name: Recombinant mouse Mlkl protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of NM_001310613 Sequence: MDKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALGRFDEVLKEANQQIEKFSKKSHIWKFVSVGNDKILFHEVNEKLRDVWEELLLLLQVYHWNTVSDVSQPASWQQEDRQDAEEDGNENMKVILMQLQISVEEINKTLKQCSLKPTQEIPQDLQIKEIPKEHLGPPWTKLKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mixed lineage kinase domain-like |
| 别名 | mixed lineage kinase domain like |
| 计算分子量 | 54kd |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | NM_001310613 |
| 基因名称 | Mlkl |
| Gene ID (NCBI) | 74568 |
| RRID | AB_3086246 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9D2Y4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MLKL antibody 30145-1-AP | Download protocol |
| WB protocol for MLKL antibody 30145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Physiol Res Trimethylamine Induced Chronic Kidney Injury by Activating the ZBP1-NLRP3 Inflammasome Pathway | ||
