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验证数据展示

  • IF Staining of HeLa using CL488-84815

    IF Staining of HeLa using CL488-84815

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Midkine antibody (CL488-84815, Clone: 241880H1 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Midkine Recombinant antibody

Midkine Recombinant Antibody for IF/ICC
Cat No. CL488-84815

产品说明书

CloneNo. 241880H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

MDK, 241880H1, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MDK Ready-To-Use IHC Kit. MDK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84815-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84815 targets Midkine in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Midkine fusion protein Ag24547 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 midkine (neurite growth-promoting factor 2)
别名 MDK, 241880H1, Amphiregulin-associated protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein
计算分子量 16 kDa
GenBank蛋白编号BC011704
基因名称 Midkine
Gene ID (NCBI) 4192
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21741
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Midkine antibody CL488-84815Download protocol
Standard Protocols
Click here to view our Standard Protocols