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验证数据展示

  • FC experiment of HeLa using 98776-3-RR

    FC experiment of HeLa using 98776-3-RR

    1x10^6 HeLa cells (left) or PC-3 cells (right) were surface stained with 0.25 ug Anti-Human Mesothelin Rabbit RecAb (98776-3-RR, Clone: 252795C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • IHC staining of human ovary cancer using 98776-3-RR

    IHC staining of human ovary cancer using 98776-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 98776-3-RR (Mesothelin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 98776-3-RR

    IHC staining of human ovary cancer using 98776-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 98776-3-RR (Mesothelin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human Mesothelin Rabbit Recombinant Antibody

Mesothelin Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98776-3-RR

产品说明书

CloneNo. 252795C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

MSLN, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor, Mesothelin, cleaved form

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98776-3-RR targets Mesothelin in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

DNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mesothelin
别名 MSLN, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor, Mesothelin, cleaved form
计算分子量69KD
GenBank蛋白编号NM_005823
基因名称 MSLN
Gene ID (NCBI) 10232
RRIDAB_3746342
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13421
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Mesothelin (MSLN) is a 40-kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71 kDa precursor protein that is processed to a 40-kDa membrane-bound protein termed mesothelin and a 31-kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

实验方案

Product Specific Protocols
FC protocol for Mesothelin antibody 98776-3-RRDownload protocol
IHC protocol for Mesothelin antibody 98776-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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