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验证数据展示

  • WB analysis of A549 using 20078-1-AP (same clone as 20078-1-PBS)

    WB analysis of A549 using 20078-1-AP (same clone as 20078-1-PBS)

    WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells. This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20078-1-AP (same clone as 20078-1-PBS)

    IP experiment of HeLa using 20078-1-AP (same clone as 20078-1-PBS)

    IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20078-1-AP (same clone as 20078-1-PBS)

    IP experiment of HeLa using 20078-1-AP (same clone as 20078-1-PBS)

    IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:1000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of mouse small intestine using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of human pancreas cancer using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of human pancreas cancer using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of human urothelial carcinoma using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of human urothelial carcinoma using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of mouse kidney using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of mouse kidney using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of mouse kidney using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of mouse kidney using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of rat kidney using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of rat kidney using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of rat small intestine using 20078-1-AP (same clone as 20078-1-PBS)

    IHC staining of rat small intestine using 20078-1-AP (same clone as 20078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IF Staining of A431 using 20078-1-AP (same clone as 20078-1-PBS)

    IF Staining of A431 using 20078-1-AP (same clone as 20078-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20078-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYSM1-Specific Polyclonal antibody, PBS Only

MYSM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20078-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MYSM1, KIAA1915, EC:3.4.19.-, Deubiquitinase MYSM1, 2A-DUB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MYSM1 Ready-To-Use IHC Kit. MYSM1 primary antibody included.

产品信息

20078-1-PBS targets MYSM1-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Myb-like, SWIRM and MPN domains 1
别名 MYSM1, KIAA1915, EC:3.4.19.-, Deubiquitinase MYSM1, 2A-DUB
计算分子量 95 kDa
观测分子量 81 kDa
GenBank蛋白编号NM_001085487
基因名称 MYSM1
Gene ID (NCBI) 114803
RRIDAB_10664926
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VVJ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

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