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  • Featured Product
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MYSM1-Specific Polyclonal antibody

MYSM1-Specific Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20078-1-AP

Print datasheet

Synonyms

2A DUB, 2ADUB, KIAA1915, MYSM1, MYSM1-Specific, RP4 592A1.1

验证数据展示

  • WB analysis of A549 using 20078-1-AP

    WB analysis of A549 using 20078-1-AP

    WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.

  • IP experiment of HeLa using 20078-1-AP

    IP experiment of HeLa using 20078-1-AP

    IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.

  • IF Staining of HeLa using 20078-1-AP

    IF Staining of HeLa using 20078-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse small intestine using 20078-1-AP

    IHC staining of mouse small intestine using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of human pancreas cancer using 20078-1-AP

    IHC staining of human pancreas cancer using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of human urothelial carcinoma using 20078-1-AP

    IHC staining of human urothelial carcinoma using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of mouse kidney using 20078-1-AP

    IHC staining of mouse kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of mouse kidney using 20078-1-AP

    IHC staining of mouse kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of rat kidney using 20078-1-AP

    IHC staining of rat kidney using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included
  • IHC staining of rat small intestine using 20078-1-AP

    IHC staining of rat small intestine using 20078-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYSM1 antibody included

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue, human spleen tissue, mouse small intestine tissue, human pancreas cancer tissue, human urothelial carcinoma tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYSM1 Ready-To-Use IHC Kit. MYSM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20078-1-AP targets MYSM1-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Myb-like, SWIRM and MPN domains 1
Synonyms 2A DUB, 2ADUB, KIAA1915, MYSM1, MYSM1-Specific, RP4 592A1.1
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberNM_001085487
Gene Symbol MYSM1
Gene ID (NCBI) 114803
RRIDAB_10664926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

实验方案

Product Specific Protocols
WB protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IHC protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IF protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
IP protocol for MYSM1-Specific antibody 20078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Discov

MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.

Authors - Xiaolin Guan
View Article