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验证数据展示

  • WB analysis using 83852-3-RR

    WB analysis using 83852-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 83852-3-RR

    IF Staining of mouse skeletal muscle using 83852-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse skeletal muscle using 83852-3-RR

    IHC staining of mouse skeletal muscle using 83852-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83852-3-RR

    Affinity and Kinetic Characterization of 83852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

MYOM3 Recombinant antibody

MYOM3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83852-3-RR

产品说明书

CloneNo. 240981F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83852-3-RR targets MYOM3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYOM3 fusion protein Ag11849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myomesin family, member 3
别名 Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9
计算分子量 162 kDa, 136 kDa
观测分子量 162 kDa
GenBank蛋白编号BC067101
基因名称 MYOM3
Gene ID (NCBI) 127294
RRIDAB_3671437
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5VTT5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.

实验方案

Product Specific Protocols
WB protocol for MYOM3 antibody 83852-3-RRDownload protocol
IHC protocol for MYOM3 antibody 83852-3-RRDownload protocol
IF protocol for MYOM3 antibody 83852-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols