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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 83852-3-RR (same clone as 83852-3-PBS)

    WB analysis using 83852-3-RR (same clone as 83852-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    IF Staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    IHC staining of mouse skeletal muscle using 83852-3-RR (same clone as 83852-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83852-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83852-3-RR

    Affinity and Kinetic Characterization of 83852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

MYOM3 Recombinant antibody, PBS Only

MYOM3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83852-3-PBS

产品说明书

CloneNo. 240981F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83852-3-PBS targets MYOM3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYOM3 fusion protein Ag11849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myomesin family, member 3
别名 Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9
计算分子量 162 kDa, 136 kDa
观测分子量 162 kDa
GenBank蛋白编号BC067101
基因名称 MYOM3
Gene ID (NCBI) 127294
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5VTT5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.