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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83807-1-RR (same clone as 83807-1-PBS)

    WB analysis using 83807-1-RR (same clone as 83807-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83807-1-RR (MYO7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83807-1-RR (same clone as 83807-1-PBS)

    IHC staining of mouse testis using 83807-1-RR (same clone as 83807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83807-1-RR (MYO7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO7A antibody included
  • IF Staining of Y79 using 83807-1-RR (same clone as 83807-1-PBS)

    IF Staining of Y79 using 83807-1-RR (same clone as 83807-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using MYO7A antibody (83807-1-RR, Clone: 240612C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83807-1-RR (same clone as 83807-1-PBS)

    FC experiment of HepG2 using 83807-1-RR (same clone as 83807-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYO7A Recombinant antibody (83807-1-RR, Clone:240612C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

MYO7A Recombinant antibody, PBS Only

MYO7A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83807-1-PBS

产品说明书

CloneNo. 240612C11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

MYU7A, MYOVIIA, myosin VIIA, DFNB2, DFNA11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYO7A Ready-To-Use IHC Kit. MYO7A primary antibody included.

产品信息

83807-1-PBS targets MYO7A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin VIIA
别名 MYU7A, MYOVIIA, myosin VIIA, DFNB2, DFNA11
计算分子量 254 kDa
观测分子量254 kDa
GenBank蛋白编号NM_000260
基因名称 MYO7A
Gene ID (NCBI) 4647
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13402
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.