×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 29996-1-AP (same clone as 29996-1-PBS)

    WB analysis using 29996-1-AP (same clone as 29996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29996-1-AP (MYO5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

    IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO5B antibody included
  • IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

    IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO5B antibody included
  • IHC staining of mouse colon using 29996-1-AP (same clone as 29996-1-PBS)

    IHC staining of mouse colon using 29996-1-AP (same clone as 29996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYO5B antibody included

MYO5B Polyclonal antibody, PBS Only

MYO5B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29996-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MYO5B Ready-To-Use IHC Kit. MYO5B primary antibody included.

产品信息

29996-1-PBS targets MYO5B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31590

Product name: Recombinant human MYO5B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 940-1114 aa of NM_001080467

Sequence: EFKTLSEQLSVTTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRKRVADLEQENALLKDEKEQLNNQILCQSKDEFAQNSVKENLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin VB
别名
计算分子量214KD
观测分子量 200 kDa
GenBank蛋白编号NM_001080467
基因名称 MYO5B
Gene ID (NCBI) 4645
RRIDAB_2935496
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULV0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).

||
New chat

Able™

正在加载，请稍候...