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验证数据展示

  • WB analysis of mouse skeletal muscle using 16052-1-AP

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 16052-1-AP

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 16052-1-AP

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 40x lens).

MYLPF Polyclonal antibody

MYLPF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16052-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16052-1-AP targets MYLPF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 MYLPF fusion protein Ag9013 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin light chain, phosphorylatable, fast skeletal muscle
别名 HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF
计算分子量 169 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012571
基因名称 MYLPF
Gene ID (NCBI) 29895
RRIDAB_2148036
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96A32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYLPF antibody 16052-1-APDownload protocol
IHC protocol for MYLPF antibody 16052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model.

Authors - Ryohei Oya
View Article
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
View Article