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验证数据展示

  • WB analysis of L02 using 83673-3-RR

    WB analysis of L02 using 83673-3-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 83673-3-RR

    WB analysis of human heart using 83673-3-RR

    human heart tissue were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83673-3-RR

    Affinity and Kinetic Characterization of 83673-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83673-3-RR against Human MYLK3 were performed. The affinity constant is below 1 pM.

MYLK3 Recombinant monoclonal antibody

MYLK3 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83673-3-RR

产品说明书

CloneNo. 240762G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, caMLCK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, human heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83673-3-RR targets MYLK3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16137

Product name: Recombinant human MYLK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC109097

Sequence: MDTKLNMLNEKVDQLLHFQEDVTEKLQSMCRDMGHLERGLHRLEASRAPGPGGADGVPHIDTQAGWPEVLELVRAMQQDAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADFLMQGRVPWRRGSPGDSPEENKERVEEEGGKPKHVLSTSGLQSDAREPGEESQKADVLEGTAERLPPIRASGLGADPAQAVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSPSLEVAPGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPRISIHIQEMDTPGEMLMTGRGSLGPTLTTEAPAAAQPGKQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin light chain kinase 3
别名 MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, caMLCK
计算分子量 819 aa, 88 kDa
观测分子量95 kDa
GenBank蛋白编号BC109097
基因名称 MYLK3
Gene ID (NCBI) 91807
RRIDAB_3671278
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ32MK0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).

实验方案

Product Specific Protocols
WB protocol for MYLK3 antibody 83673-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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