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验证数据展示

  • WB analysis using 22281-1-AP (same clone as 22281-1-PBS)

    WB analysis using 22281-1-AP (same clone as 22281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

    IF Staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH6 antibody (22281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

    IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH6 antibody included
  • IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

    IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH6 antibody included
  • IF Staining of HSkMC using 22281-1-AP (same clone as 22281-1-PBS)

    IF Staining of HSkMC using 22281-1-AP (same clone as 22281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HSkMC cells using MYH6 antibody (22281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYH6 Polyclonal antibody, PBS Only

MYH6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 22281-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Myosin 6, MYHCA, MyHC-alpha, Myosin heavy chain 6, Myosin-6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYH6 Ready-To-Use IHC Kit. MYH6 primary antibody included.

产品信息

22281-1-PBS targets MYH6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 6, cardiac muscle, alpha
别名 Myosin 6, MYHCA, MyHC-alpha, Myosin heavy chain 6, Myosin-6
计算分子量 224 kDa
观测分子量 200-220 kDa
GenBank蛋白编号NM_002471
基因名称 MYH6
Gene ID (NCBI) 4624
RRIDAB_2736822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

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