验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy MYH6 Ready-To-Use IHC Kit. MYH6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22281-1-AP targets MYH6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken, zebrafish, bovine, goat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy chain 6, cardiac muscle, alpha |
| 别名 | Myosin 6, MYHCA, MyHC-alpha, Myosin heavy chain 6, Myosin-6 |
| 计算分子量 | 224 kDa |
| 观测分子量 | 200-220 kDa |
| GenBank蛋白编号 | NM_002471 |
| 基因名称 | MYH6 |
| Gene ID (NCBI) | 4624 |
| RRID | AB_2736822 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH6 antibody 22281-1-AP | Download protocol |
| IHC protocol for MYH6 antibody 22281-1-AP | Download protocol |
| WB protocol for MYH6 antibody 22281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. | ||
Cell Stem Cell Epi-Allele elicits compensatory expression of the non-targeted allele and prevents haploinsufficiency in dominant genetic diseases | ||
Nat Commun Fluorofenidone ameliorates pressure overload-induced heart failure by stabilizing SERCA2a in male mice. | ||
Redox Biol iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy | ||
Theranostics Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes | ||
Smart Med Mechanistic Insights Into NFIX-Mediated DNA Recognition and Transcriptional Regulation in Skeletal Muscle. |
