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验证数据展示

  • WB analysis using 86157-3-RR

    WB analysis using 86157-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 86157-3-RR

    IHC staining of mouse cerebellum using 86157-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH10 antibody included
  • WB analysis using 86157-3-RR

    WB analysis using 86157-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 86157-3-RR

    IF Staining of mouse cerebellum using 86157-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using MYH10 antibody (86157-3-RR, Clone: 250791D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYH10 Recombinant antibody

MYH10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86157-3-RR

产品说明书

CloneNo. 250791D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, mouse cerebellum tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86157-3-RR targets MYH10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYH10 fusion protein Ag16113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, heavy chain 10, non-muscle
别名 Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b
计算分子量 1985 aa, 230 kDa
观测分子量 229 kDa
GenBank蛋白编号BC150634
基因名称 MYH10
Gene ID (NCBI) 4628
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35580
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

实验方案

Product Specific Protocols
WB protocol for MYH10 antibody 86157-3-RRDownload protocol
IHC protocol for MYH10 antibody 86157-3-RRDownload protocol
IF protocol for MYH10 antibody 86157-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols