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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH10 antibody included
  • WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    IF Staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using MYH10 antibody (86157-3-RR, Clone: 250791D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

MYH10 Recombinant antibody, PBS Only

MYH10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86157-3-PBS

产品说明书

CloneNo. 250791D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.

产品信息

86157-3-PBS targets MYH10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYH10 fusion protein Ag16113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, heavy chain 10, non-muscle
别名 Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b
计算分子量 1985 aa, 230 kDa
观测分子量 229 kDa
GenBank蛋白编号BC150634
基因名称 MYH10
Gene ID (NCBI) 4628
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35580
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.