MYD88 Monoclonal antibody

MYD88 Monoclonal Antibody for WB, ELISA
Cat No. 66660-1-Ig

产品说明书

CloneNo. 1B10E12

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse and More (1)

应用

WB, ELISA and More (1)

MYD88, MYD88D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHSC-T6 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, SP2/0 cells
Planning an IHC experiment? We recommend our IHCeasy MYD88 Ready-To-Use IHC Kit. MYD88 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66660-1-Ig targets MYD88 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, rat, mouse
文献引用反应性human, mouse
免疫原 MYD88 fusion protein Ag19770 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myeloid differentiation primary response gene (88)
别名 MYD88, MYD88D
计算分子量 33 kDa
观测分子量 33-36 kDa
GenBank蛋白编号BC013589
基因名称 MYD88
Gene ID (NCBI) 4615
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99836
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

Myeloid differentiation primary response 88 (MYD88) is an adapter protein critical to the innate and adaptive immune response.

 

What is the molecular weight of MYD88?

The molecular weight of MYD88 is 33 kDa.

 

What is the cellular localization of MYD88?

The subcellular localization of MYD88 is largely confined to the cytoplasm as condensed forms or aggregated structures.

 

What is the role of MYD88 in the IL-1R signaling pathway?

MYD88 plays a major role in the inflammatory signaling pathways downstream of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) families. MYD88 links these receptors to IL-1R-associated kinases (IRAK), such as IRAK1 and IRAK2, via protein-protein interactions. The C-terminal TIR domain of MYD88 mediates the interaction with the receptors, whereas the N-terminal death domain of MYD88 associates with IRAK family members (PMID: 25580251). MYD88 acts via its intermediate domain to phosphorylate and thus activate IRAK1, IRAK2, IRF7, and TRAF6 to trigger NF-kappa-B signaling and cytokine secretion as part of the inflammatory response (PMID: 19679662).

 

What is MYD88's involvement in disease?

Defects in MYD88 due to deficiency of the protein leads to recurrent pyogenic bacterial infections, including invasive pneumococcal disease. Patients usually die between 1 and 11 months of age, but surviving patients are otherwise healthy with normal resistance to other microbes (PMID: 18669862). Mutations in the MYD88 gene also lead to the development of cancers such as lymphoma (PMID: 21179087) and some autoimmune disorders like ulcerative colitis (PMID: 24189845).


实验方案

Product Specific Protocols
WB protocol for MYD88 antibody 66660-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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mouseWB

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Catalpol relieved angiotensin II-induced blood–brain barrier destruction via inhibiting the TLR4 pathway in brain endothelial cells

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Life Sci

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Oncol Rep

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