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验证数据展示

  • WB analysis of human heart using 12022-1-AP (same clone as 12022-1-PBS)

    WB analysis of human heart using 12022-1-AP (same clone as 12022-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

  • WB analysis using 12022-1-AP (same clone as 12022-1-PBS)

    WB analysis using 12022-1-AP (same clone as 12022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

    IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYCBP antibody included
  • IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

    IHC staining of human pancreas cancer using 12022-1-AP (same clone as 12022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYCBP antibody included
  • IHC staining of mouse colon using 12022-1-AP (same clone as 12022-1-PBS)

    IHC staining of mouse colon using 12022-1-AP (same clone as 12022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYCBP antibody included
  • IF Staining of HepG2 using 12022-1-AP (same clone as 12022-1-PBS)

    IF Staining of HepG2 using 12022-1-AP (same clone as 12022-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYCBP antibody (12022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12022-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYCBP Polyclonal antibody, PBS Only

MYCBP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12022-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYCBP Ready-To-Use IHC Kit. MYCBP primary antibody included.

产品信息

12022-1-PBS targets MYCBP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2651

Product name: Recombinant human MYCBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC008686

Sequence: MAHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 c-myc binding protein
别名 c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1
计算分子量 103 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC008686
基因名称 MYCBP
Gene ID (NCBI) 26292
RRIDAB_2148722
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99417
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.

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