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MTSS1L Polyclonal antibody

MTSS1L Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27832-1-AP

Print datasheet

Synonyms

ABBA 1, metastasis suppressor 1 like, MTSS1 like protein, MTSS1L

验证数据展示

  • IP experiment of Jurkat using 27832-1-AP

    IP experiment of Jurkat using 27832-1-AP

    IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.

  • WB analysis using 27832-1-AP

    WB analysis using 27832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27832-1-AP

    IHC staining of mouse brain using 27832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27832-1-AP targets MTSS1L in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen MTSS1L fusion protein Ag27216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metastasis suppressor 1-like
Synonyms ABBA 1, metastasis suppressor 1 like, MTSS1 like protein, MTSS1L
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC002770
Gene Symbol MTSS1L
Gene ID (NCBI) 92154
RRIDAB_2880986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ765P7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTSS1L antibody 27832-1-APDownload protocol
IHC protocol for MTSS1L antibody 27832-1-APDownload protocol
IF protocol for MTSS1L antibody 27832-1-APDownload protocol
IP protocol for MTSS1L antibody 27832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

MicroRNA-23a promotes colorectal cancer cell migration and proliferation by targeting at MARK1.

Authors - Xiaoli Tang
View Article